› 3d9j

X-ray diffraction
1.6Å resolution

Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II

Released:
DOI: 10.2210/pdb3d9j/pdb
PDB entry 3d9j coloured by chain, front view.
PDB entry 3d9j coloured by chain, side view.
PDB entry 3d9j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the carboxyl-terminal domain of RNA polymerase II.
Becker R, Loll B, Meinhart A
J Biol Chem 283 22659-69 (2008)
PMID: 18550522

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194027 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SR-related and CTD-associated factor 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UPN6 (Residues: 1-136; Coverage: 11%)
Gene names: CCAP7, KIAA1116, RBM16, SCAF8
Sequence domains: CID domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

3 bound ligands:
Ligand NH4 12 x NH4
Ligand SO4 10 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43
Unit cell:
a: 57.62Å b: 57.62Å c: 107.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.206
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The RNA polymerase II CTD coordinates transcription and RNA processing.
Hsin et al. (2012)
12 more

1 mention without citation

N-terminal truncation of an isolated human IgG1 CH2 domain significantly increases its stability and aggregation resistance.
Gong et al. (2013)