› 3deb

X-ray diffraction
1.95Å resolution

Crystal Structure of apo form (Zinc removed) of the Botulinum Neurotoxin Type C Light Chain

Released:
DOI: 10.2210/pdb3deb/pdb
PDB entry 3deb coloured by chain, front view.
PDB entry 3deb coloured by chain, side view.
PDB entry 3deb coloured by chain, top view.
Source organism: Clostridium botulinum
Entry authors: Rawat R, Kumaran D, Swaminathan S

Function and Biology Details

Reaction catalysed: 
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Botulinum neurotoxin C light chain Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 51.51 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: P18640 (Residues: 1-430; Coverage: 33%)
Sequence domains: Clostridial neurotoxin zinc protease
Structure domains: Metalloproteases ("zincins"), catalytic domain like

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand CA 1 x CA
Ligand ACT 1 x ACT
Ligand FMT 7 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3121
Unit cell:
a: 106.74Å b: 106.74Å c: 140.24Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.194 0.2
Expression system: Escherichia coli

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