› 3dos

X-ray diffraction
2.4Å resolution

Crystal structure of the complex of the Caf1M chaperone with the mini-fiber of two Caf1 subunits (Caf1:Caf1), carrying the Thr7Phe and Ala9Val mutations in the Gd donor strand

Released:
DOI: 10.2210/pdb3dos/pdb
PDB entry 3dos coloured by chain, front view.
PDB entry 3dos coloured by chain, side view.
PDB entry 3dos coloured by chain, top view.
Source organism: Yersinia pestis
Entry authors: Fooks LJ, Yu X, Moslehi-Mohebi E, Tischenko V, Knight SD, MacIntyre S, Zavialov AV

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150882 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein caf1M Chains: A, D
Molecule details ›
Chains: A, D
Length: 235 amino acids
Theoretical weight: 26.33 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: P26926 (Residues: 24-258; Coverage: 99%)
Gene names: Y1098, YPMT1.82, YP_pMT084, caf1M
Sequence domains:
Structure domains: Immunoglobulins
F1 capsule antigen Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 149 amino acids
Theoretical weight: 15.65 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: P26948 (Residues: 22-170; Coverage: 100%)
Gene names: Y1100, YPMT1.84, YP_pMT082, caf1
Sequence domains: Caf1 Capsule antigen
Structure domains: Fimbrial-type adhesion domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P212121
Unit cell:
a: 72.234Å b: 91.181Å c: 166.024Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.264
Expression system: Escherichia coli

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