› 3du4

X-ray diffraction
2.2Å resolution

Crystal structure of 7-keto-8-aminopelargonic acid bound 7,8-diaminopelargonic acid synthase in bacillus subtilis

Released:
DOI: 10.2210/pdb3du4/pdb
PDB entry 3du4 coloured by chain, front view.
PDB entry 3du4 coloured by chain, side view.
PDB entry 3du4 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase .
Dey S, Lane JM, Lee RE, Rubin EJ, Sacchettini JC
Biochemistry 49 6746-60 (2010)
PMID: 20565114

Function and Biology Details

Reaction catalysed: 
L-lysine + 8-amino-7-oxononanoate = (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-Lysine--8-amino-7-oxononanoate transaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 448 amino acids
Theoretical weight: 50.19 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P53555 (Residues: 1-448; Coverage: 100%)
Gene names: BSU30230, bioA, bioK
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand KAP 2 x KAP
1 modified residue:
Ligand CSX 2 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 57.692Å b: 105.133Å c: 75.063Å
α: 90° β: 105.19° γ: 90°
R-values:
R R work R free
0.204 0.201 0.26
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Closing in on complete pathways of biotin biosynthesis.
Lin et al. (2011)
6 more

3 mentions without citation

Structural characterization of the Mycobacterium tuberculosis biotin biosynthesis enzymes 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase .
Dey et al. (2010)
2 more