› 3edx

X-ray diffraction
2.4Å resolution

Crystal structure of the W215A/E217A mutant of murine thrombin

Released:
DOI: 10.2210/pdb3edx/pdb
PDB entry 3edx coloured by chain, front view.
PDB entry 3edx coloured by chain, side view.
PDB entry 3edx coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Gandhi PS, Page MJ, Chen Z, Bush-pelc L, Di Cera E

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148522 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thrombin light chain Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 44 amino acids
Theoretical weight: 5.11 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P19221 (Residues: 317-360; Coverage: 7%)
Gene names: Cf2, F2
Sequence domains: Thrombin light chain
Structure domains: Thrombin light chain domain
Thrombin heavy chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 258 amino acids
Theoretical weight: 29.8 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P19221 (Residues: 361-618; Coverage: 43%)
Gene names: Cf2, F2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 97.716Å b: 105.76Å c: 176.811Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.243
Expression system: Cricetulus griseus

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