› 3eir

X-ray diffraction
2.1Å resolution

Crystal structure of CHBP, a Cif Homologue from Burkholderia pseudomallei

Released:
DOI: 10.2210/pdb3eir/pdb
PDB entry 3eir coloured by chain, front view.
PDB entry 3eir coloured by chain, side view.
PDB entry 3eir coloured by chain, top view.
Source organism: Burkholderia pseudomallei
Primary publication:
A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.
Yao Q, Cui J, Zhu Y, Wang G, Hu L, Long C, Cao R, Liu X, Huang N, Chen S, Liu L, Shao F
Proc Natl Acad Sci U S A 106 3716-21 (2009)
PMID: 19225106

Function and Biology Details

Reaction catalysed: 
Protein L-glutamine + H(2)O = protein L-glutamate + NH(3)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179059 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-glutamine deamidase Cif Chains: A, B
Molecule details ›
Chains: A, B
Length: 281 amino acids
Theoretical weight: 31.31 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q63KH5 (Residues: 48-328; Coverage: 86%)
Gene names: BPSS1385, cif
Sequence domains: Cycle inhibiting factor (CIF)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 54.783Å b: 79.916Å c: 117.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.193 0.254
Expression system: Escherichia coli

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Citations

18 review citations

Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis.
Galyov et al. (2010)
17 more

2 mentions without citation

A bacterial type III effector family uses the papain-like hydrolytic activity to arrest the host cell cycle.
Yao et al. (2009)
1 more