› 3enb

X-ray diffraction
1.85Å resolution

Crystal Structure of PRP8 core domain IV

Released:
DOI: 10.2210/pdb3enb/pdb
PDB entry 3enb coloured by chain, front view.
PDB entry 3enb coloured by chain, side view.
PDB entry 3enb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural elucidation of a PRP8 core domain from the heart of the spliceosome.
Ritchie DB, Schellenberg MJ, Gesner EM, Raithatha SA, Stuart DT, Macmillan AM
Nat Struct Mol Biol 15 1199-205 (2008)
PMID: 18836455

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-processing-splicing factor 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6P2Q9 (Residues: 1769-1990; Coverage: 10%)
Gene names: PRPC8, PRPF8
Sequence domains: PRP8 domain IV core
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 75.397Å b: 78.062Å c: 93.575Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.243
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

The spliceosome: design principles of a dynamic RNP machine.
Wahl et al. (2009)
12 more

1 mention without citation

A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.
Schellenberg et al. (2013)