› 3f2w

X-ray diffraction
3.45Å resolution

Crystal structure of the FMn riboswitch bound to FMN, Ba2+ soak.

Released:
DOI: 10.2210/pdb3f2w/pdb
PDB entry 3f2w coloured by chain, front view.
PDB entry 3f2w coloured by chain, side view.
PDB entry 3f2w coloured by chain, top view.
Source organism: Fusobacterium nucleatum
Primary publication:
Coenzyme recognition and gene regulation by a flavin mononucleotide riboswitch.
Serganov A, Huang L, Patel DJ
Nature 458 233-7 (2009)
PMID: 19169240

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196664 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
FMN riboswitch Chain: X
Molecule details ›
Chain: X
Length: 112 nucleotides
Theoretical weight: 36.45 KDa
Source organism: Fusobacterium nucleatum
Expression system: Not provided
Sequence domains: FMN riboswitch aptamer (RFN element)

Ligands and Environments

3 bound ligands:
Ligand FMN 1 x FMN
Ligand MG 7 x MG
Ligand BA 16 x BA
2 modified residues:
Ligand GTP 1 x GTP
Ligand CCC 1 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3121
Unit cell:
a: 71.003Å b: 71.003Å c: 139.745Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.181 0.217
Expression system: Not provided

Quick links

Citations

46 review citations

The noncoding RNA revolution-trashing old rules to forge new ones.
Cech et al. (2014)
45 more

3 mentions without citation

Using RNA Sequence and Structure for the Prediction of Riboswitch Aptamer: A Comprehensive Review of Available Software and Tools.
Antunes et al. (2017)
2 more