› 3f53

X-ray diffraction
2Å resolution

Crystal structure of Toxoplasma gondii micronemal protein 1 bound to 2F-3'SiaLacNAc

Released:
DOI: 10.2210/pdb3f53/pdb
PDB entry 3f53 coloured by chain, front view.
PDB entry 3f53 coloured by chain, side view.
PDB entry 3f53 coloured by chain, top view.
Source organism: Toxoplasma gondii RH
Primary publication:
Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.
Garnett JA, Liu Y, Leon E, Allman SA, Friedrich N, Saouros S, Curry S, Soldati-Favre D, Davis BG, Feizi T, Matthews S
Protein Sci 18 1935-47 (2009)
PMID: 19593815

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126398 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Micronemal protein 1 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.27 KDa
Source organism: Toxoplasma gondii RH
Expression system: Escherichia coli
UniProt:
  • Canonical: O00834 (Residues: 17-262; Coverage: 56%)
Gene name: MIC1
Sequence domains: Sialic-acid binding micronemal adhesive repeat
Structure domains: Actin; Chain A, domain 4

Ligands and Environments

Carbohydrate polymer : NEW Components: 2FG, SIA
Carbohydrate polymer
3 bound ligands:
Ligand CL 1 x CL
Ligand ACT 8 x ACT
Ligand GOL 5 x GOL
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P43212
Unit cell:
a: 66.361Å b: 66.361Å c: 172.371Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.191
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Using glycan microarrays to understand immunity.
Arthur et al. (2014)
6 more

1 mention without citation

Detailed insights from microarray and crystallographic studies into carbohydrate recognition by microneme protein 1 (MIC1) of Toxoplasma gondii.
Garnett et al. (2009)