› 3f6d

X-ray diffraction
1.7Å resolution

Crystal Structure of a Genetically Modified Delta Class GST (adGSTD4-4) from Anopheles dirus, F123A, in Complex with S-Hexyl Glutathione

Released:
DOI: 10.2210/pdb3f6d/pdb
PDB entry 3f6d coloured by chain, front view.
PDB entry 3f6d coloured by chain, side view.
PDB entry 3f6d coloured by chain, top view.
Source organism: Anopheles dirus
Primary publication:
Structural contributions of delta class glutathione transferase active-site residues to catalysis.
Wongsantichon J, Robinson RC, Ketterman AJ
Biochem J 428 25-32 (2010)
PMID: 20196771

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutathione transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.93 KDa
Source organism: Anopheles dirus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GN60 (Residues: 1-219; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTX 2 x GTX
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P3221
Unit cell:
a: 49.692Å b: 49.692Å c: 272.943Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.173 0.202
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Insect glutathione transferases.
Ketterman et al. (2011)
8 other articles

1 mention without citation

Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.
Niegowski et al. (2014)