› 3f72

X-ray diffraction
2.31Å resolution

Crystal Structure of the Staphylococcus aureus pI258 CadC Metal Binding Site 2 Mutant

Released:
DOI: 10.2210/pdb3f72/pdb
PDB entry 3f72 coloured by chain, front view.
PDB entry 3f72 coloured by chain, side view.
PDB entry 3f72 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Role of bound Zn(II) in the CadC Cd(II)/Pb(II)/Zn(II)-responsive repressor.
Kandegedara A, Thiyagarajan S, Kondapalli KC, Stemmler TL, Rosen BP
J Biol Chem 284 14958-65 (2009)
PMID: 19286656

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cadmium resistance transcriptional regulatory protein CadC Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 122 amino acids
Theoretical weight: 13.63 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20047 (Residues: 1-122; Coverage: 100%)
Gene name: cadC
Sequence domains: Bacterial regulatory protein, arsR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand NA 5 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P43
Unit cell:
a: 89.471Å b: 89.471Å c: 148.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.288
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
4 more

3 mentions without citation

Entropy redistribution controls allostery in a metalloregulatory protein.
Capdevila et al. (2017)
2 more