› 3f7p

X-ray diffraction
2.75Å resolution

Crystal structure of a complex between integrin beta4 and plectin

Released:
DOI: 10.2210/pdb3f7p/pdb
PDB entry 3f7p coloured by chain, front view.
PDB entry 3f7p coloured by chain, side view.
PDB entry 3f7p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the interaction between integrin alpha6beta4 and plectin at the hemidesmosomes.
de Pereda JM, Lillo MP, Sonnenberg A
EMBO J 28 1180-90 (2009)
PMID: 19242489

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-147643 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 33.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15149 (Residues: 120-403; Coverage: 6%)
  • Best match: Q15149-2 (Residues: 1-293)
Gene names: PLEC, PLEC1
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain
Integrin beta-4 Chains: C, D, E
Molecule details ›
Chains: C, D, E
Length: 248 amino acids
Theoretical weight: 27.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16144 (Residues: 1126-1369; Coverage: 14%)
  • Best match: P16144-2 (Residues: 1126-1370)
Gene name: ITGB4
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:
Ligand EDO 2 x EDO
Ligand PEG 1 x PEG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3221
Unit cell:
a: 107.25Å b: 107.25Å c: 203.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.204 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Type VII collagen: the anchoring fibril protein at fault in dystrophic epidermolysis bullosa.
Chung et al. (2010)
16 more

6 mentions without citation

Advances and perspectives of the architecture of hemidesmosomes: lessons from structural biology.
de Pereda et al. (2009)
5 more