› 3fce

X-ray diffraction
1.9Å resolution

Crystal Structure of Bacillus cereus D-alanyl Carrier Protein Ligase DltA in Complex with ATP: Implications for Adenylation Mechanism

Released:
DOI: 10.2210/pdb3fce/pdb
PDB entry 3fce coloured by chain, front view.
PDB entry 3fce coloured by chain, side view.
PDB entry 3fce coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) in complex with ATP.
Osman KT, Du L, He Y, Luo Y
J Mol Biol 388 345-55 (2009)
PMID: 19324056

Function and Biology Details

Reaction catalysed: 
(1a) ATP + D-alanine = (D-alanyl)adenylate + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182523 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanine--D-alanyl carrier protein ligase Chain: A
Molecule details ›
Chain: A
Length: 512 amino acids
Theoretical weight: 57.63 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81G39 (Residues: 1-504; Coverage: 100%)
Gene names: BC_1372, dltA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 61.4Å b: 87.4Å c: 57.4Å
α: 90° β: 114° γ: 90°
R-values:
R R work R free
0.207 0.207 0.252
Expression system: Escherichia coli

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Citations

8 review citations

Lipoteichoic acid synthesis and function in gram-positive bacteria.
Percy et al. (2014)
7 more

7 mentions without citation

Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.
Gulick AM. (2009)
6 more