› 3fhg

X-ray diffraction
1.9Å resolution

Crystal structure of Sulfolobus solfataricus 8-oxoguanine DNA glycosylase (SsOgg)

Released:
DOI: 10.2210/pdb3fhg/pdb
PDB entry 3fhg coloured by chain, front view.
PDB entry 3fhg coloured by chain, side view.
PDB entry 3fhg coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Crystal structures of two archaeal 8-oxoguanine DNA glycosylases provide structural insight into guanine/8-oxoguanine distinction.
Faucher F, Duclos S, Bandaru V, Wallace SS, Doublié S
Structure 17 703-12 (2009)
PMID: 19446526

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
8-oxoguanine DNA glycosylase/AP lyase Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 24.29 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZK2 (Residues: 1-207; Coverage: 100%)
Gene names: SSO0904, ogg
Sequence domains: 8-oxoguanine DNA glycosylase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P6
Unit cell:
a: 110.96Å b: 110.96Å c: 34.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.207 0.248
Expression system: Escherichia coli

Quick links

Citations

12 citation in other articles

Roles of the Y-family DNA polymerase Dbh in accurate replication of the Sulfolobus genome at high temperature.
Sakofsky et al. (2012)
11 more

3 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
2 more