› 3fji

X-ray diffraction
2.55Å resolution

Crystal structure of K12V/C83I/C117V mutant of Human acidic fibroblast growth factor

Released:
DOI: 10.2210/pdb3fji/pdb
PDB entry 3fji coloured by chain, front view.
PDB entry 3fji coloured by chain, side view.
PDB entry 3fji coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of conserved cysteine in the fibroblast growth factor family: evidence for a vestigial half-cystine.
Lee J, Blaber M
J Mol Biol 393 128-39 (2009)
PMID: 19683004

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138603 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibroblast growth factor 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 146 amino acids
Theoretical weight: 16.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05230 (Residues: 16-155; Coverage: 90%)
Gene names: FGF1, FGFA
Sequence domains: Fibroblast growth factor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 50.75Å b: 108.076Å c: 67.675Å
α: 90° β: 103.73° γ: 90°
R-values:
R R work R free
0.212 0.212 0.263
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Protein comparability assessments and potential applicability of high throughput biophysical methods and data visualization tools to compare physical stability profiles.
Alsenaidy et al. (2014)
1 more