› 3fwu

X-ray diffraction
1.8Å resolution

Crystal structure of Leishmania major MIF1

Released:
DOI: 10.2210/pdb3fwu/pdb
PDB entry 3fwu coloured by chain, front view.
PDB entry 3fwu coloured by chain, side view.
PDB entry 3fwu coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Structures of Leishmania major orthologues of macrophage migration inhibitory factor.
Richardson JM, Morrison LS, Bland ND, Bruce S, Coombs GH, Mottram JC, Walkinshaw MD
Biochem Biophys Res Commun 380 442-8 (2009)
PMID: 19187777

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-dopachrome isomerase Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.7 KDa
Source organism: Leishmania major
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4Q413 (Residues: 1-113; Coverage: 100%)
Gene names: LMJF_33_1740, MIF1
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: R3
Unit cell:
a: 52.321Å b: 52.321Å c: 97.94Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.2 0.264
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The non-mammalian MIF superfamily.
Sparkes et al. (2017)
2 more