› 3g3o

X-ray diffraction
2.1Å resolution

Crystal structure of the cytoplasmic tunnel domain in yeast Vtc2p

Released:
DOI: 10.2210/pdb3g3o/pdb
PDB entry 3g3o coloured by chain, front view.
PDB entry 3g3o coloured by chain, side view.
PDB entry 3g3o coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase.
Hothorn M, Neumann H, Lenherr ED, Wehner M, Rybin V, Hassa PO, Uttenweiler A, Reinhardt M, Schmidt A, Seiler J, Ladurner AG, Herrmann C, Scheffzek K, Mayer A
Science 324 513-6 (2009)
PMID: 19390046

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155093 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar transporter chaperone complex subunit 2 Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 45.44 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P43585 (Residues: 183-553; Coverage: 45%)
Gene names: PHM1, VTC2, YFL004W
Sequence domains: VTC domain
Structure domains: VTC, catalytic tunnel domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 71.2Å b: 74.59Å c: 80.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.236
Expression system: Escherichia coli

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Citations

47 review citations

Polyphosphate: an ancient molecule that links platelets, coagulation, and inflammation.
Morrissey et al. (2012)
46 more