› 3g7u

X-ray diffraction
1.75Å resolution

Crystal structure of putative DNA modification methyltransferase encoded within prophage Cp-933R (E.coli)

Released:
DOI: 10.2210/pdb3g7u/pdb
PDB entry 3g7u coloured by chain, front view.
PDB entry 3g7u coloured by chain, side view.
PDB entry 3g7u coloured by chain, top view.
Source organism: Escherichia coli O157:H7 str. EDL933
Entry authors: Patskovsky Y, Ramagopal UA, Toro R, Gilmore M, Iizuka M, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-570808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosine-specific methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 376 amino acids
Theoretical weight: 42.3 KDa
Source organism: Escherichia coli O157:H7 str. EDL933
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8X8S5 (Residues: 2-366; Coverage: 95%)
Gene name: ECs_1953
Sequence domains: C-5 cytosine-specific DNA methylase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3121
Unit cell:
a: 79.436Å b: 79.436Å c: 126.539Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.183 0.219
Expression system: Escherichia coli

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