› 3gb3

X-ray diffraction
1.75Å resolution

X-ray structure of genetically encoded photosensitizer KillerRed in native form

Released:
DOI: 10.2210/pdb3gb3/pdb
PDB entry 3gb3 coloured by chain, front view.
PDB entry 3gb3 coloured by chain, side view.
PDB entry 3gb3 coloured by chain, top view.
Source organism: Anthomedusae sp. DC-2005
Primary publication:
Structural basis for phototoxicity of the genetically encoded photosensitizer KillerRed.
Pletnev S, Gurskaya NG, Pletneva NV, Lukyanov KA, Chudakov DM, Martynov VI, Popov VO, Kovalchuk MV, Wlodawer A, Dauter Z, Pletnev V
J Biol Chem 284 32028-39 (2009)
PMID: 19737938

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174039 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KillerRed Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.41 KDa
Source organism: Anthomedusae sp. DC-2005
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2TCH5 (Residues: 1-237; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand CRQ 2 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 72.016Å b: 73.682Å c: 78.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.244
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
22 more

5 mentions without citation

Genetically encoded tools for measuring and manipulating metabolism.
Choe et al. (2022)
4 more