› 3gb6

X-ray diffraction
2Å resolution

Structure of Giardia fructose-1,6-biphosphate aldolase D83A mutant in complex with fructose-1,6-bisphosphate

Released:
DOI: 10.2210/pdb3gb6/pdb
PDB entry 3gb6 coloured by chain, front view.
PDB entry 3gb6 coloured by chain, side view.
PDB entry 3gb6 coloured by chain, top view.
Source organism: Giardia intestinalis
Primary publication:
Structural insights into the substrate binding and stereoselectivity of giardia fructose-1,6-bisphosphate aldolase.
Galkin A, Li Z, Li L, Kulakova L, Pal LR, Dunaway-Mariano D, Herzberg O
Biochemistry 48 3186-96 (2009)
PMID: 19236002

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108573 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-bisphosphate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 35.22 KDa
Source organism: Giardia intestinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: A8B2U2 (Residues: 1-323; Coverage: 100%)
Gene names: FBPA, GL50803_0011043, fba
Sequence domains: Fructose-bisphosphate aldolase class-II
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand P6F 2 x P6F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 56.5Å b: 67.18Å c: 172.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.254
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
2 more

5 mentions without citation

Fructose degradation in the haloarchaeon Haloferax volcanii involves a bacterial type phosphoenolpyruvate-dependent phosphotransferase system, fructose-1-phosphate kinase, and class II fructose-1,6-bisphosphate aldolase.
Pickl et al. (2012)
4 more