› 3gbr

X-ray diffraction
2.25Å resolution

Anthranilate phosphoribosyl-transferase (TRPD) double mutant D83G F149S from S. solfataricus

Released:
DOI: 10.2210/pdb3gbr/pdb
PDB entry 3gbr coloured by chain, front view.
PDB entry 3gbr coloured by chain, side view.
PDB entry 3gbr coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Activation of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus by removal of magnesium inhibition and acceleration of product release .
Schlee S, Deuss M, Bruning M, Ivens A, Schwab T, Hellmann N, Mayans O, Sterner R
Biochemistry 48 5199-209 (2009)
PMID: 19385665

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-D-ribosyl)-anthranilate + diphosphate = anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156171 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anthranilate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 37.5 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P50384 (Residues: 1-345; Coverage: 100%)
Gene names: SSO0890, trpD
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PRP 2 x PRP
Ligand MN 4 x MN
Ligand PEG 2 x PEG
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 61.945Å b: 65.993Å c: 84.262Å
α: 90° β: 98.81° γ: 90°
R-values:
R R work R free
0.194 0.192 0.26
Expression system: Escherichia coli

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Citations

1 review citation

Structure, mechanism and inhibition of anthranilate phosphoribosyltransferase.
Scully et al. (2023)
4 other articles