› 3gce

X-ray diffraction
2Å resolution

Ferredoxin of carbazole 1,9a-dioxygenase from Nocardioides aromaticivorans IC177

Released:
DOI: 10.2210/pdb3gce/pdb
PDB entry 3gce coloured by chain, front view.
PDB entry 3gce coloured by chain, side view.
PDB entry 3gce coloured by chain, top view.
Source organism: Nocardioides aromaticivorans
Primary publication:
Specific Interactions between the ferredoxin and terminal oxygenase components of a class IIB Rieske nonheme iron oxygenase, carbazole 1,9a-dioxygenase.
Inoue K, Ashikawa Y, Umeda T, Abo M, Katsuki J, Usami Y, Noguchi H, Fujimoto Z, Terada T, Yamane H, Nojiri H
J Mol Biol 392 436-51 (2009)
PMID: 19616558

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin component of carbazole 1,9a-dioxygenase Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 13.15 KDa
Source organism: Nocardioides aromaticivorans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2HWH6 (Residues: 1-115; Coverage: 100%)
Gene name: carAc
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

1 bound ligand:
Ligand FES 1 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P41212
Unit cell:
a: 50.116Å b: 50.116Å c: 82.224Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.189
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural and molecular genetic analyses of the bacterial carbazole degradation system.
Nojiri H. (2012)
15 other articles

1 mention without citation

The α- and β-Subunit Boundary at the Stem of the Mushroom-Like α3β3-Type Oxygenase Component of Rieske Non-Heme Iron Oxygenases Is the Rieske-Type Ferredoxin-Binding Site.
Tsai et al. (2022)