› 3gcx

X-ray diffraction
2.7Å resolution

PCSK9:EGFA (pH 7.4)

Released:
DOI: 10.2210/pdb3gcx/pdb
PDB entry 3gcx coloured by chain, front view.
PDB entry 3gcx coloured by chain, side view.
PDB entry 3gcx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Antagonism of secreted PCSK9 increases low density lipoprotein receptor expression in HepG2 cells.
McNutt MC, Kwon HJ, Chen C, Chen JR, Horton JD, Lagace TA
J Biol Chem 284 10561-70 (2009)
PMID: 19224862

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134047 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proprotein convertase subtilisin/kexin type 9 Chain: P
Molecule details ›
Chain: P
Length: 100 amino acids
Theoretical weight: 11.28 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NBP7 (Residues: 53-152; Coverage: 15%)
Gene names: NARC1, PCSK9, PSEC0052
Sequence domains: Peptidase inhibitor I9
Structure domains: Peptidase S8 propeptide/proteinase inhibitor I9
Proprotein convertase subtilisin/kexin type 9 Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 57.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NBP7 (Residues: 153-692; Coverage: 82%)
Gene names: NARC1, PCSK9, PSEC0052
Sequence domains:
Structure domains:
Low-density lipoprotein receptor Chain: E
Molecule details ›
Chain: E
Length: 83 amino acids
Theoretical weight: 9.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01130 (Residues: 314-393; Coverage: 10%)
Gene name: LDLR
Structure domains: Laminin

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 116.047Å b: 116.047Å c: 133.629Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.261
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

35 review citations

The biology and therapeutic targeting of the proprotein convertases.
Seidah et al. (2012)
34 more

7 mentions without citation

Antagonism of secreted PCSK9 increases low density lipoprotein receptor expression in HepG2 cells.
McNutt et al. (2009)
6 more