Function and Biology Details
Reaction catalysed:
Toluene + NADH + O(2) = 4-methylphenol + NAD(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assemblies composition:
Assembly name:
Toluene-4-monooxygenase system, (preferred)
PDBe Complex ID:
PDB-CPX-169416 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Toluene-4-monooxygenase system, hydroxylase component subunit alpha
Molecule details ›
Chains: A, D
Length: 500 amino acids
Theoretical weight: 58.14 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
Length: 500 amino acids
Theoretical weight: 58.14 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
Q00456 (Residues: 1-500; Coverage: 100%)
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
Toluene-4-monooxygenase system, hydroxylase component subunit beta
Molecule details ›
Chains: B, F
Length: 327 amino acids
Theoretical weight: 38.43 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
Length: 327 amino acids
Theoretical weight: 38.43 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
- Canonical: Q00460 (Residues: 1-327; Coverage: 100%)
Sequence domains: Methane/Phenol/Alkene Hydroxylase
Structure domains: Ribonucleotide Reductase, subunit A
Toluene-4-monooxygenase system, hydroxylase component subunit gamma
Molecule details ›
Chains: C, G
Length: 84 amino acids
Theoretical weight: 9.6 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Toluene-4-monooxygenase system protein B (TmoB)
Structure domains: TmoB-like
Length: 84 amino acids
Theoretical weight: 9.6 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
- Canonical: Q00457 (Residues: 1-84; Coverage: 100%)
Sequence domains: Toluene-4-monooxygenase system protein B (TmoB)
Structure domains: TmoB-like
Toluene-4-monooxygenase system, effector component
Molecule details ›
Chains: E, H
Length: 103 amino acids
Theoretical weight: 11.63 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: MmoB/DmpM family
Structure domains: Monooxygenase component MmoB/DmpM
Length: 103 amino acids
Theoretical weight: 11.63 KDa
Source organism: Pseudomonas mendocina
Expression system: Escherichia coli BL21
UniProt:
- Canonical: Q00459 (Residues: 1-103; Coverage: 100%)
Sequence domains: MmoB/DmpM family
Structure domains: Monooxygenase component MmoB/DmpM
