› 3geq

X-ray diffraction
2.2Å resolution

Structural basis for the chemical rescue of Src kinase activity

Released:
DOI: 10.2210/pdb3geq/pdb
PDB entry 3geq coloured by chain, front view.
PDB entry 3geq coloured by chain, side view.
PDB entry 3geq coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Comparative analysis of mutant tyrosine kinase chemical rescue.
Muratore KE, Seeliger MA, Wang Z, Fomina D, Neiswinger J, Havranek JJ, Baker D, Kuriyan J, Cole PA
Biochemistry 48 3378-86 (2009)
PMID: 19260709

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 286 amino acids
Theoretical weight: 32.64 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00523 (Residues: 251-533; Coverage: 53%)
Gene name: SRC
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PP2 2 x PP2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 59.649Å b: 63.462Å c: 83.86Å
α: 90° β: 91.45° γ: 90°
R-values:
R R work R free
0.226 0.226 0.273
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Catalytic mechanisms and regulation of protein kinases.
Wang et al. (2014)
3 more

7 mentions without citation

Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics.
Ferrando et al. (2012)
6 more