› 3gko

X-ray diffraction
1.6Å resolution

Crystal structure of urate oxydase using surfactant Poloxamer 188 as a New Crystallizing Agent

Released:
DOI: 10.2210/pdb3gko/pdb
PDB entry 3gko coloured by chain, front view.
PDB entry 3gko coloured by chain, side view.
PDB entry 3gko coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Surfactant Poloxamer 188 as a New Crystallizing Agent for Urate Oxidase
Giffard M, Delfosse V, Sciara G, Mayer C, Cambillau C, El Hajji M, Castro B, Bonnete F
Cryst Growth Des 9 4199-4206 (2009)

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 34.2 KDa
Source organism: Aspergillus flavus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q00511 (Residues: 2-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

2 bound ligands:
Ligand AZA 1 x AZA
Ligand K 2 x K
1 modified residue:
Ligand SAC 1 x SAC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: I222
Unit cell:
a: 80.063Å b: 95.284Å c: 104.534Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.179 0.199
Expression system: Saccharomyces cerevisiae

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