› 3gn5

X-ray diffraction
2.15Å resolution

Structure of the E. coli protein MqsA (YgiT/b3021)

Released:
DOI: 10.2210/pdb3gn5/pdb
PDB entry 3gn5 coloured by chain, front view.
PDB entry 3gn5 coloured by chain, side view.
PDB entry 3gn5 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Three dimensional structure of the MqsR:MqsA complex: a novel TA pair comprised of a toxin homologous to RelE and an antitoxin with unique properties.
Brown BL, Grigoriu S, Kim Y, Arruda JM, Davenport A, Wood TK, Peti W, Page R
OpenAccess logo PLoS Pathog 5 e1000706 (2009)
PMID: 20041169

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175148 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Antitoxin MqsA Chain: A
Molecule details ›
Chain: A
Length: 133 amino acids
Theoretical weight: 14.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46864 (Residues: 1-131; Coverage: 100%)
Gene names: JW2989, b3021, mqsA, ygiT
Sequence domains: Antitoxin component of bacterial toxin-antitoxin system, MqsA
Structure domains:
Antitoxin MqsA Chain: B
Molecule details ›
Chain: B
Length: 133 amino acids
Theoretical weight: 14.94 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46864 (Residues: 1-131; Coverage: 100%)
Gene names: JW2989, b3021, mqsA, ygiT
Sequence domains: Antitoxin component of bacterial toxin-antitoxin system, MqsA
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 62.054Å b: 30.965Å c: 75.542Å
α: 90° β: 106.6° γ: 90°
R-values:
R R work R free
0.185 0.182 0.233
Expression system: Escherichia coli

Quick links

Citations

36 review citations

Toxin-antitoxin systems in bacteria and archaea.
Yamaguchi et al. (2011)
35 more

8 mentions without citation

Three dimensional structure of the MqsR:MqsA complex: a novel TA pair comprised of a toxin homologous to RelE and an antitoxin with unique properties.
Brown et al. (2009)
7 more