› 3h70

X-ray diffraction
1.6Å resolution

Crystal structure of o-succinylbenzoic acid synthetase from staphylococcus aureus Complexed with mg in the active site

Released:
DOI: 10.2210/pdb3h70/pdb
PDB entry 3h70 coloured by chain, front view.
PDB entry 3h70 coloured by chain, side view.
PDB entry 3h70 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family.
Odokonyero D, Sakai A, Patskovsky Y, Malashkevich VN, Fedorov AA, Bonanno JB, Fedorov EV, Toro R, Agarwal R, Wang C, Ozerova ND, Yew WS, Sauder JM, Swaminathan S, Burley SK, Almo SC, Glasner ME
Proc Natl Acad Sci U S A 111 8535-40 (2014)
PMID: 24872444

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176062 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
o-succinylbenzoate synthase Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.23 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53635 (Residues: 2-333; Coverage: 100%)
Gene names: ACR74_14530, C0102_15075, CNH36_09570, EIG96_05600, EP54_13350, EQ90_13570, GO814_01450, GO942_01815, GQX37_07885, LB359_09135, NCTC13131_06039, SAMEA1029512_02810, SAMEA1029528_02832, SAMEA1029536_02813, SAMEA2078260_02777, SAMEA2078588_02633, SAMEA2080344_02798, SAMEA2081063_02831, SAMEA4008575_02897, SAMEA70146418_02956, menC, menc
Sequence domains: Enolase C-terminal domain-like
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 59.081Å b: 40.271Å c: 72.386Å
α: 90° β: 92.75° γ: 90°
R-values:
R R work R free
0.202 0.2 0.221
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biosynthesis and applications of prenylquinones.
Kawamukai M. (2018)
2 more

1 mention without citation

Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family.
Odokonyero et al. (2014)