› 3h7i

X-ray diffraction
1.5Å resolution

Structure of the metal-free D132N T4 RNase H

Released:
DOI: 10.2210/pdb3h7i/pdb
PDB entry 3h7i coloured by chain, front view.
PDB entry 3h7i coloured by chain, side view.
PDB entry 3h7i coloured by chain, top view.
Source organism: Escherichia virus T4
Entry authors: Tomanicek SJ, Mueser TC

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease H Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 35.61 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P13319 (Residues: 1-305; Coverage: 100%)
Gene names: 33.2, das, rnh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 36.011Å b: 85.858Å c: 57.26Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.181 0.18 0.213
Expression system: Escherichia coli

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