› 3hae

X-ray diffraction
2.9Å resolution

Rational development of high-affinity T-cell receptor-like antibodies

Released:
DOI: 10.2210/pdb3hae/pdb
PDB entry 3hae coloured by chain, front view.
PDB entry 3hae coloured by chain, side view.
PDB entry 3hae coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rational development of high-affinity T-cell receptor-like antibodies.
Stewart-Jones G, Wadle A, Hombach A, Shenderov E, Held G, Fischer E, Kleber S, Nuber N, Stenner-Liewen F, Bauer S, McMichael A, Knuth A, Abken H, Hombach AA, Cerundolo V, Jones EY, Renner C
Proc Natl Acad Sci U S A 106 5784-8 (2009)
PMID: 19307587

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-209029 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, D, J, P
Molecule details ›
Chains: A, D, J, P
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E, K, Q
Molecule details ›
Chains: B, E, K, Q
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Cancer/testis antigen 1 Chains: C, F, M, R
Molecule details ›
Chains: C, F, M, R
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78358 (Residues: 157-164; Coverage: 4%)
Gene names: CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, LAGE2, LAGE2A, LAGE2B
Antibody light chain Chains: G, L, N, S
Molecule details ›
Chains: G, L, N, S
Length: 212 amino acids
Theoretical weight: 22.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Antibody heavy chain Chains: H, I, O, T
Molecule details ›
Chains: H, I, O, T
Length: 220 amino acids
Theoretical weight: 22.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 70.794Å b: 105.596Å c: 256.451Å
α: 90° β: 92.26° γ: 90°
R-values:
R R work R free
0.205 0.201 0.283
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

29 review citations

The basic principles of chimeric antigen receptor design.
Sadelain et al. (2013)
28 more

5 mentions without citation

Targeting the MHC Ligandome by Use of TCR-Like Antibodies.
Høydahl et al. (2019)
4 more