› 3hgv

X-ray diffraction
2.3Å resolution

Structure of Phenazine Antibiotic Biosynthesis Protein

Released:
DOI: 10.2210/pdb3hgv/pdb
PDB entry 3hgv coloured by chain, front view.
PDB entry 3hgv coloured by chain, side view.
PDB entry 3hgv coloured by chain, top view.
Source organism: Pantoea agglomerans
Primary publication:
Structure of the D-alanylgriseoluteic acid biosynthetic protein EhpF, an atypical member of the ANL superfamily of adenylating enzymes.
Bera AK, Atanasova V, Gamage S, Robinson H, Parsons JF
Acta Crystallogr D Biol Crystallogr 66 664-72 (2010)
PMID: 20516619

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EhpF Chains: A, B
Molecule details ›
Chains: A, B
Length: 369 amino acids
Theoretical weight: 41.56 KDa
Source organism: Pantoea agglomerans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8GPH0 (Residues: 1-366; Coverage: 100%)
Gene name: ehpF

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 68.836Å b: 110.143Å c: 112.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.249
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent insights into the diversity, frequency and ecological roles of phenazines in fluorescent Pseudomonas spp.
Mavrodi et al. (2013)
1 more

1 mention without citation

Structure of the D-alanylgriseoluteic acid biosynthetic protein EhpF, an atypical member of the ANL superfamily of adenylating enzymes.
Bera et al. (2010)