› 3hrv

X-ray diffraction
1.5Å resolution

Crystal structure of TcpA, a Type IV pilin from Vibrio cholerae El Tor biotype

Released:
DOI: 10.2210/pdb3hrv/pdb
PDB entry 3hrv coloured by chain, front view.
PDB entry 3hrv coloured by chain, side view.
PDB entry 3hrv coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Vibrio cholerae El Tor TcpA crystal structure and mechanism for pilus-mediated microcolony formation.
Lim MS, Ng D, Zong Z, Arvai AS, Taylor RK, Tainer JA, Craig L
Mol Microbiol 77 755-70 (2010)
PMID: 20545841

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toxin coregulated pilin Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 19.74 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60153 (Residues: 54-224; Coverage: 76%)
Gene names: VC_0828, tcpA
Sequence domains: Toxin-coregulated pilus subunit TcpA
Structure domains: TcpA-like pilin

Ligands and Environments

2 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I212121
Unit cell:
a: 62.767Å b: 94.752Å c: 128.422Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.224
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Type IV pilin proteins: versatile molecular modules.
Giltner et al. (2012)
3 more

3 mentions without citation

Vibrio cholerae El Tor TcpA crystal structure and mechanism for pilus-mediated microcolony formation.
Lim et al. (2010)
2 more