› 3hv0

X-ray diffraction
2.42Å resolution

Tryptophanyl-tRNA synthetase from Cryptosporidium parvum

Released:
DOI: 10.2210/pdb3hv0/pdb
PDB entry 3hv0 coloured by chain, front view.
PDB entry 3hv0 coloured by chain, side view.
PDB entry 3hv0 coloured by chain, top view.
Source organism: Cryptosporidium parvum Iowa II
Primary publication:
Crystal structures of three protozoan homologs of tryptophanyl-tRNA synthetase.
Merritt EA, Arakaki TL, Gillespie R, Napuli AJ, Kim JE, Buckner FS, Van Voorhis WC, Verlinde CL, Fan E, Zucker F, Hol WG
Mol Biochem Parasitol 177 20-8 (2011)
PMID: 21255615

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tryptophan--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 45.28 KDa
Source organism: Cryptosporidium parvum Iowa II
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5CYP8 (Residues: 206-593; Coverage: 65%)
Gene name: cgd7_1490
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TRP 2 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 58.32Å b: 85.22Å c: 73.11Å
α: 90° β: 101.91° γ: 90°
R-values:
R R work R free
0.229 0.227 0.273
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Aminoacyl-tRNA synthetases as drug targets in eukaryotic parasites.
Pham et al. (2014)
4 more

2 mentions without citation

An appended domain results in an unusual architecture for malaria parasite tryptophanyl-tRNA synthetase.
Khan et al. (2013)
1 more