› 3hyr

X-ray diffraction
2.2Å resolution

Structural Insight into G Protein Coupling and Regulation of Fe2+ Membrane Transport

Released:
DOI: 10.2210/pdb3hyr/pdb
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PDB entry 3hyr coloured by chain, front view.
PDB entry 3hyr coloured by chain, side view.
PDB entry 3hyr coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of GDP release and gating in G protein coupled Fe2+ transport.
Guilfoyle A, Maher MJ, Rapp M, Clarke R, Harrop S, Jormakka M
EMBO J 28 2677-85 (2009)
PMID: 19629046

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-152627 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe(2+) transporter FeoB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 270 amino acids
Theoretical weight: 29.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P33650 (Residues: 1-270; Coverage: 35%)
Gene names: JW3372, b3409, feoB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 146.12Å b: 84.44Å c: 66.24Å
α: 90° β: 108.46° γ: 90°
R-values:
R R work R free
0.228 0.225 0.287
Expression system: Escherichia coli

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Citations

3 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
2 more

6 mentions without citation

Ins and Outs: Recent Advancements in Membrane Protein-Mediated Prokaryotic Ferrous Iron Transport.
Brown et al. (2021)
5 more

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