› 3hyt

X-ray diffraction
2.74Å resolution

Structural Basis of GDP Release and Gating in G Protein Coupled Fe2+ Transport

Released:
DOI: 10.2210/pdb3hyt/pdb
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PDB entry 3hyt coloured by chain, front view.
PDB entry 3hyt coloured by chain, side view.
PDB entry 3hyt coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of GDP release and gating in G protein coupled Fe2+ transport.
Guilfoyle A, Maher MJ, Rapp M, Clarke R, Harrop S, Jormakka M
EMBO J 28 2677-85 (2009)
PMID: 19629046

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe(2+) transporter FeoB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 270 amino acids
Theoretical weight: 29.71 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P33650 (Residues: 1-270; Coverage: 35%)
Gene names: JW3372, b3409, feoB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AGO 3 x AGO
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 76Å b: 46.41Å c: 120.07Å
α: 90° β: 94.95° γ: 90°
R-values:
R R work R free
0.23 0.227 0.294
Expression system: Escherichia coli

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Citations

2 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
1 more

8 mentions without citation

Toward a mechanistic understanding of Feo-mediated ferrous iron uptake.
Sestok et al. (2018)
7 more

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