› 3i80

X-ray diffraction
2.25Å resolution

Protein Tyrosine Phosphatase 1B - Transition state analog for the second catalytic step

Released:
DOI: 10.2210/pdb3i80/pdb
PDB entry 3i80 coloured by chain, front view.
PDB entry 3i80 coloured by chain, side view.
PDB entry 3i80 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the reaction of protein-tyrosine phosphatase 1B: crystal structures for transition state analogs of both catalytic steps.
Brandão TA, Hengge AC, Johnson SJ
J Biol Chem 285 15874-83 (2010)
PMID: 20236928

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 37.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

3 bound ligands:
Ligand VO4 1 x VO4
Ligand TRS 1 x TRS
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 87.84Å b: 87.84Å c: 103.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.235
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Vanadium in Biological Action: Chemical, Pharmacological Aspects, and Metabolic Implications in Diabetes Mellitus.
Treviño et al. (2019)
12 more

18 mentions without citation

The metal face of protein tyrosine phosphatase 1B.
Bellomo et al. (2016)
17 more