› 3ibr

X-ray diffraction
2.97Å resolution

Crystal Structure of P. aeruginosa Bacteriophytochrome Photosensory Core Module Mutant Q188L in the Mixed Pr/Pfr State

Released:
DOI: 10.2210/pdb3ibr/pdb
PDB entry 3ibr coloured by chain, front view.
PDB entry 3ibr coloured by chain, side view.
PDB entry 3ibr coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Conformational differences between the Pfr and Pr states in Pseudomonas aeruginosa bacteriophytochrome.
Yang X, Kuk J, Moffat K
Proc Natl Acad Sci U S A 106 15639-44 (2009)
PMID: 19720999

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chains: A, B
Molecule details ›
Chains: A, B
Length: 505 amino acids
Theoretical weight: 57.37 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HWR3 (Residues: 1-497; Coverage: 68%)
Gene names: PA4117, bphP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BLA 2 x BLA
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 108.902Å b: 108.902Å c: 188.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.233 0.307
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Structure and function of plant photoreceptors.
Möglich et al. (2010)
14 more

2 mentions without citation

Phytochromes in Agrobacterium fabrum.
Lamparter et al. (2021)
1 more