› 3ihf

X-ray diffraction
2.28Å resolution

Crystal structure of mouse Bcl-xl mutant (R139A) at pH 5.0

Released:
DOI: 10.2210/pdb3ihf/pdb
PDB entry 3ihf coloured by chain, front view.
PDB entry 3ihf coloured by chain, side view.
PDB entry 3ihf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural insights into mouse anti-apoptotic Bcl-xl reveal affinity for Beclin 1 and gossypol.
Priyadarshi A, Roy A, Kim KS, Kim EE, Hwang KY
Biochem Biophys Res Commun 394 515-21 (2010)
PMID: 20206602

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bcl-2-like protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 196 amino acids
Theoretical weight: 21.97 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q64373 (Residues: 1-196; Coverage: 84%)
  • Best match: Q64373-2 (Residues: 1-125)
Gene names: Bcl2l, Bcl2l1, Bclx
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P1
Unit cell:
a: 35.353Å b: 56.525Å c: 96.443Å
α: 89.98° β: 89.93° γ: 89.82°
R-values:
R R work R free
0.197 0.193 0.266
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

The Bcl-2-Beclin 1 interaction in (-)-gossypol-induced autophagy versus apoptosis in prostate cancer cells.
Lian et al. (2010)
5 more

1 mention without citation

The Structural Biology of Bcl-xL.
Lee et al. (2019)