› 3ilu

X-ray diffraction
2Å resolution

Crystal structure of the AMPA subunit GluR2 bound to the allosteric modulator, hydroflumethiazide

Released:
DOI: 10.2210/pdb3ilu/pdb
PDB entry 3ilu coloured by chain, front view.
PDB entry 3ilu coloured by chain, side view.
PDB entry 3ilu coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Probing the allosteric modulator binding site of GluR2 with thiazide derivatives.
Ptak CP, Ahmed AH, Oswald RE
Biochemistry 48 8594-602 (2009)
PMID: 19673491

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 2 Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 258 amino acids
Theoretical weight: 28.81 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19491 (Residues: 414-527, 653-794; Coverage: 30%)
Gene names: GluA2, Glur2, Gria2
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:
Ligand GLU 3 x GLU
Ligand HFZ 3 x HFZ
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Unit cell:
a: 47.289Å b: 114.194Å c: 163.732Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.226
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Functional insights from glutamate receptor ion channel structures.
Kumar et al. (2013)
7 more

2 mentions without citation

Probing the allosteric modulator binding site of GluR2 with thiazide derivatives.
Ptak et al. (2009)
1 more