› 3ipc

X-ray diffraction
1.3Å resolution

Structure of ATU2422-GABA F77A mutant receptor in complex with leucine

Released:
DOI: 10.2210/pdb3ipc/pdb
PDB entry 3ipc coloured by chain, front view.
PDB entry 3ipc coloured by chain, side view.
PDB entry 3ipc coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
A conserved mechanism of GABA binding and antagonism is revealed by structure-function analysis of the periplasmic binding protein Atu2422 in Agrobacterium tumefaciens.
Planamente S, Vigouroux A, Mondy S, Nicaise M, Faure D, Moréra S
J Biol Chem 285 30294-303 (2010)
PMID: 20630861

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-binding protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 37.44 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7CX36 (Residues: 24-372; Coverage: 100%)
Gene name: Atu2422
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand LEU 1 x LEU
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 119.77Å b: 43.03Å c: 66.46Å
α: 90° β: 109.76° γ: 90°
R-values:
R R work R free
0.159 0.158 0.186
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Quorum quenching: role in nature and applied developments.
Grandclément et al. (2016)
5 more

2 mentions without citation

A conserved mechanism of GABA binding and antagonism is revealed by structure-function analysis of the periplasmic binding protein Atu2422 in Agrobacterium tumefaciens.
Planamente et al. (2010)
1 more