› 3is3

X-ray diffraction
1.48Å resolution

Crystal structure of 17beta-Hydroxysteroid dehydrogenase (Apo form) from fungus Cochliobolus lunatus

Released:
DOI: 10.2210/pdb3is3/pdb
PDB entry 3is3 coloured by chain, front view.
PDB entry 3is3 coloured by chain, side view.
PDB entry 3is3 coloured by chain, top view.
Source organism: Curvularia lunata
Entry authors: Cassetta A, Lamba D, Krastanova I

Function and Biology Details

Reaction catalysed: 
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-131691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17beta-hydroxysteroid dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 28.94 KDa
Source organism: Curvularia lunata
Expression system: Escherichia coli
UniProt:
  • Canonical: O93874 (Residues: 1-270; Coverage: 100%)
Gene name: 17HSDcl
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand PEG 1 x PEG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: I4122
Unit cell:
a: 67.914Å b: 67.914Å c: 268.347Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.188
Expression system: Escherichia coli

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