› 3iwz

X-ray diffraction
2.3Å resolution

The c-di-GMP Responsive Global Regulator CLP Links Cell-Cell Signaling to Virulence Gene Expression in Xanthomonas campestris

Released:
DOI: 10.2210/pdb3iwz/pdb
PDB entry 3iwz coloured by chain, front view.
PDB entry 3iwz coloured by chain, side view.
PDB entry 3iwz coloured by chain, top view.
Source organism: Xanthomonas campestris pv. campestris
Primary publication:
The cAMP receptor-like protein CLP is a novel c-di-GMP receptor linking cell-cell signaling to virulence gene expression in Xanthomonas campestris.
Chin KH, Lee YC, Tu ZL, Chen CH, Tseng YH, Yang JM, Ryan RP, McCarthy Y, Dow JM, Wang AH, Chou SH
J Mol Biol 396 646-62 (2010)
PMID: 20004667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRP-like protein Clp Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 230 amino acids
Theoretical weight: 25.75 KDa
Source organism: Xanthomonas campestris pv. campestris
Expression system: Escherichia coli
UniProt:
  • Canonical: P22260 (Residues: 1-230; Coverage: 100%)
Gene names: XCC0472, clp
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 67.69Å b: 67.69Å c: 110.37Å
α: 90° β: 104.2° γ: 90°
R-values:
R R work R free
0.244 0.244 0.305
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
27 more

3 mentions without citation

Diversity of Cyclic Di-GMP-Binding Proteins and Mechanisms.
Chou et al. (2016)
2 more