› 3k31

X-ray diffraction
1.8Å resolution

Crystal structure of eonyl-(acyl-carrier-protein) reductase from anaplasma phagocytophilum in complex with nad at 1.9a resolution

Released:
DOI: 10.2210/pdb3k31/pdb
PDB entry 3k31 coloured by chain, front view.
PDB entry 3k31 coloured by chain, side view.
PDB entry 3k31 coloured by chain, top view.
Source organism: Anaplasma phagocytophilum str. HZ
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-173611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase [NADH] Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 31.91 KDa
Source organism: Anaplasma phagocytophilum str. HZ
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2GKM8 (Residues: 1-275; Coverage: 100%)
Gene names: APH_0473, fabI
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand EDO 3 x EDO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21212
Unit cell:
a: 80.78Å b: 89.73Å c: 79.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.175
Expression system: Escherichia coli BL21(DE3)

Quick links