› 3k42

X-ray diffraction
2.3Å resolution

Crystal structure of sCD-MPR mutant E19Q/K137M pH 7.0

Released:
DOI: 10.2210/pdb3k42/pdb
PDB entry 3k42 coloured by chain, front view.
PDB entry 3k42 coloured by chain, side view.
PDB entry 3k42 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Intermonomer interactions are essential for lysosomal enzyme binding by the cation-dependent mannose 6-phosphate receptor.
Olson LJ, Sun G, Bohnsack RN, Peterson FC, Dahms NM, Kim JJ
Biochemistry 49 236-46 (2010)
PMID: 19928875

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145846 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cation-dependent mannose-6-phosphate receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 17.44 KDa
Source organism: Bos taurus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P11456 (Residues: 29-182; Coverage: 61%)
Gene name: M6PR
Sequence domains: Mannose-6-phosphate receptor
Structure domains: Mannose-6-phosphate receptor binding domain

Ligands and Environments

3 bound ligands:
Ligand NAG 2 x NAG
Ligand 1GP 2 x 1GP
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: I4
Unit cell:
a: 102.582Å b: 102.582Å c: 100.145Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.249
Expression system: Komagataella pastoris

Quick links

Citations

3 review citations

Mechanisms and concepts paving the way towards a complete transport cycle of plant vacuolar sorting receptors.
De Marcos Lousa et al. (2012)
2 more

2 mentions without citation

Intermonomer interactions are essential for lysosomal enzyme binding by the cation-dependent mannose 6-phosphate receptor.
Olson et al. (2010)
1 more