› 3k7q

X-ray diffraction
2.05Å resolution

Crystal structure of substrate-bound 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans

Released:
DOI: 10.2210/pdb3k7q/pdb
PDB entry 3k7q coloured by chain, front view.
PDB entry 3k7q coloured by chain, side view.
PDB entry 3k7q coloured by chain, top view.
Source organism: Paenarthrobacter nicotinovorans
Primary publication:
Crystal structure analysis of free and substrate-bound 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans.
Kachalova GS, Bourenkov GP, Mengesdorf T, Schenk S, Maun HR, Burghammer M, Riekel C, Decker K, Bartunik HD
J Mol Biol 396 785-99 (2010)
PMID: 20006620

Function and Biology Details

Reaction catalysed: 
(1a) (S)-6-hydroxynicotine + O(2) = 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188104 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-6-hydroxynicotine oxidase Chain: X
Molecule details ›
Chain: X
Length: 431 amino acids
Theoretical weight: 47.21 KDa
Source organism: Paenarthrobacter nicotinovorans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93NH4 (Residues: 1-425; Coverage: 100%)
Gene name: 6-hlno
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:
Ligand HNL 1 x HNL
Ligand GP7 1 x GP7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P432
Unit cell:
a: 162.988Å b: 162.988Å c: 162.988Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.25
Expression system: Escherichia coli

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Citations

2 review citations

Advances on the Transfer of Lipids by Lipid Transfer Proteins.
Wong et al. (2017)
1 more