› 3kcs

X-ray diffraction
1.5Å resolution

Crystal structure of PAmCherry1 in the dark state

Released:
DOI: 10.2210/pdb3kcs/pdb
PDB entry 3kcs coloured by chain, front view.
PDB entry 3kcs coloured by chain, side view.
PDB entry 3kcs coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states.
Subach FV, Malashkevich VN, Zencheck WD, Xiao H, Filonov GS, Almo SC, Verkhusha VV
Proc Natl Acad Sci U S A 106 21097-102 (2009)
PMID: 19934036

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 234 amino acids
Theoretical weight: 26.78 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: D1MPT3 (Residues: 1-236; Coverage: 100%)
Gene names: PAmCherry, PAmCherry1
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 1 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I222
Unit cell:
a: 69.174Å b: 83.745Å c: 99.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.206
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Advances in fluorescence labeling strategies for dynamic cellular imaging.
Dean et al. (2014)
14 more

1 mention without citation

Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states.
Subach et al. (2009)