› 3kd7

X-ray diffraction
2.85Å resolution

Designed TPR module (CTPR390) in complex with its peptide-ligand (Hsp90 peptide)

Released:
DOI: 10.2210/pdb3kd7/pdb
PDB entry 3kd7 coloured by chain, front view.
PDB entry 3kd7 coloured by chain, side view.
PDB entry 3kd7 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a designed tetratricopeptide repeat module in complex with its peptide ligand.
Cortajarena AL, Wang J, Regan L
FEBS J 277 1058-66 (2010)
PMID: 20089039

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163704 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
CTPR390 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 125 amino acids
Theoretical weight: 14.37 KDa
Source organism: unidentified
Expression system: Escherichia coli
Structure domains: Tetratricopeptide repeat domain
DSCR1 Chains: G, H, I, J, K
Molecule details ›
Chains: G, H, I, J, K
Length: 6 amino acids
Theoretical weight: 648 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9H2A1 (Residues: 1-5; Coverage: 17%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: R3
Unit cell:
a: 100.674Å b: 100.674Å c: 161.574Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.271 0.271 0.283
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

DARPins and other repeat protein scaffolds: advances in engineering and applications.
Boersma et al. (2011)
3 more

6 mentions without citation

NextGen protein design.
Sawyer et al. (2013)
5 more