› 3kep

X-ray diffraction
1.82Å resolution

Crystal structure of the autoproteolytic domain from the nuclear pore complex component NUP145 from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3kep/pdb
PDB entry 3kep coloured by chain, front view.
PDB entry 3kep coloured by chain, side view.
PDB entry 3kep coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of the autoproteolytic domain from the Saccharomyces cerevisiae nuclear pore complex component, Nup145.
Sampathkumar P, Ozyurt SA, Do J, Bain KT, Dickey M, Rodgers LA, Gheyi T, Sali A, Kim SJ, Phillips J, Pieper U, Fernandez-Martinez J, Franke JD, Martel A, Tsuruta H, Atwell S, Thompson DA, Emtage JS, Wasserman SR, Rout MP, Sauder JM, Burley SK
Proteins 78 1992-8 (2010)
PMID: 20310066

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoporin NUP145N Chains: A, B
Molecule details ›
Chains: A, B
Length: 174 amino acids
Theoretical weight: 20.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49687 (Residues: 442-605; Coverage: 13%)
Gene names: NUP145, RAT10, YGL092W
Sequence domains: Nucleoporin autopeptidase
Structure domains: Peptidase S59, nucleoporin

Ligands and Environments

1 bound ligand:
Ligand EDO 17 x EDO
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P43212
Unit cell:
a: 91.037Å b: 91.037Å c: 108.394Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.244
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Structure of the Nuclear Pore Complex (An Update).
Lin et al. (2019)
1 more

4 mentions without citation

Integrative structural modeling with small angle X-ray scattering profiles.
Schneidman-Duhovny et al. (2012)
3 more