› 3kkw

X-ray diffraction
1.41Å resolution

Crystal structure of His-tagged form of PA4794 protein

Released:
DOI: 10.2210/pdb3kkw/pdb
PDB entry 3kkw coloured by chain, front view.
PDB entry 3kkw coloured by chain, side view.
PDB entry 3kkw coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Double trouble-Buffer selection and His-tag presence may be responsible for nonreproducibility of biomedical experiments.
Majorek KA, Kuhn ML, Chruszcz M, Anderson WF, Minor W
Protein Sci 23 1359-68 (2014)
PMID: 25044180

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190989 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.38 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HV14 (Residues: 1-160; Coverage: 100%)
Gene name: PA4794
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand EDO 17 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 57.534Å b: 76.145Å c: 39.454Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Role of Buffers in Protein Formulations.
Zbacnik et al. (2017)
8 more

3 mentions without citation

Double trouble-Buffer selection and His-tag presence may be responsible for nonreproducibility of biomedical experiments.
Majorek et al. (2014)
2 more