3kll

X-ray diffraction
2Å resolution

Crystal structure of Lactobacillus reuteri N-terminally truncated glucansucrase GTF180-maltose complex

Released:

Function and Biology Details

Reaction catalysed:
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
dextransucrase Chain: A
Molecule details ›
Chain: A
Length: 1039 amino acids
Theoretical weight: 117.77 KDa
Source organism: Limosilactobacillus reuteri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SBN3 (Residues: 742-1772; Coverage: 60%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P1
Unit cell:
a: 58.165Å b: 65.85Å c: 82.317Å
α: 73.44° β: 78.57° γ: 86.34°
R-values:
R R work R free
0.165 0.163 0.199
Expression system: Escherichia coli